Environmental comparative study of biosurfactants production and optimization using bacterial strains isolated from Egyptian oil fields

Biosurfactants have recently gained popularity because they numerous benefits over chemical synthetic surfactants, including higher biodegradability, lower toxicity, foaming, environmental compatibility, and effective properties under harsh conditions. This study aimed to produce biosurfactants by selected bacterial strains isolated from Egyptian oil fields improve recovery investigate their aspects for microbial enhanced recovery. The were incubated in a new proposed nutrient medium H with optimum surface emulsification activities. Stability studies conducted examine the tolerance of produced reservoir Core flooding tests performed potential enhancing risk assessment was if there are any possible threats strains. Results showed that Bacillus licheniformis subtilis could reached maximum activity after 24 h incubation reducing tension 71.8 mN/m 27.13 25.74 mN/m, interfacial against kerosene 48.4 1.27 0.38 at critical micelle concentration 0.06 g/l 0.04 g/l, respectively. significant crude indices 50.2% 63.7%, High stability observed high temperatures long-time period more than 60% activities maintained wide range pH salinity. It also found 31.41–39.35% additional be recovered biosurfactants. Finally, environmentally safe, no occur MEOR.